製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RCC1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of human RCC1 (NP_001260.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa or 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • 配列類似性
    Contains 7 RCC1 repeats.
  • 翻訳後修飾
    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • 細胞内局在
    Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

画像

  • All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution

    Lane 1 : Recombinant Human RCC1 protein (ab90625) at 0.01 µg
    Lane 2 : Recombinant Human RCC1 protein (ab90625) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes
  • All lanes : Anti-RCC1 antibody (ab70749) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

参考文献

ab70749 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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