製品の概要

  • 製品名Anti-RCC1 antibody
    RCC1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RCC1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of human RCC1 (NP_001260.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa or 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • 配列類似性Contains 7 RCC1 repeats.
  • 翻訳後修飾N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • 細胞内局在Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Anti-RCC1 antibody 画像

  • All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution

    Lane 1 : Recombinant Human RCC1 protein (ab90625) at 0.01 µg
    Lane 2 : Recombinant Human RCC1 protein (ab90625) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes
  • All lanes : Anti-RCC1 antibody (ab70749) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-RCC1 antibody (ab70749) 使用論文

ab70749 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"