Anti-RCC1 抗体 (ab70749)
Key features and details
- Goat polyclonal to RCC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RCC1 antibody
RCC1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RCC1 -
由来種
Goat -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human RCC1 (N terminal).
Database link: P18754 -
ポジティブ・コントロール
- WB: Whole cell lysate from HeLa, 293T cells; IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70749の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 1-2 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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特記事項 |
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IP
Use at 1-2 µg/mg of lysate. |
WB
1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ターゲット情報
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機能
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. -
配列類似性
Contains 7 RCC1 repeats. -
翻訳後修飾
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. -
細胞内局在
Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 1104 Human
- Omim: 179710 Human
- SwissProt: P18754 Human
- Unigene: 469723 Human
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別名
- Cell cycle regulatory protein antibody
- CHC 1 antibody
- CHC1 antibody
see all
画像
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All lanes : Anti-RCC1 antibody (ab70749) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293T whole ell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 45 kDaChemiluminescence with an exposure time of 30 seconds.
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Detection of human RCC1 by western blot of immunoprecipitates.
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: Anti-RCC1 antibody ab70749 used for IP at 6 µg per reaction
Lane 2: Anti-RCC1 antibody, ab245594
Lane 3: IgG Control
For blotting immunoprecipitated RCC1, ab70749 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution
Lane 1 :Recombinant Human RCC1 protein (ab90625) at 0.01 µg
Lane 2 :Recombinant Human RCC1 protein (ab90625) at 0.1 µg
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution (Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70749 は論文での使用が確認できていません。