The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1.
Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
Western Blot showing Lane 2: ab70196 at 0.04µg/ml staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70196 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 1: an irrelevant antibody was used for immunoprecipitation.
Chemiluminescence with an exposure time of 30 seconds.