製品の概要
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製品名Anti-RBPJK antibody - ChIP Grade
RBPJK 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to RBPJK - ChIP Grade
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由来種Rabbit
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アプリケーション適用あり: IP, ChIP, EMSA, WB, ICC/IFmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RBPJK.
(Peptides available as ab26893)
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ポジティブ･コントロール
- This antibody gave a positive signal in Human Pancreas tissue lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab25949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use a concentration of 5 µg/ml. | |
| ChIP | Use at an assay dependent concentration. PubMed: 20661424 | |
| EMSA | Use at an assay dependent concentration. PubMed: 21991380 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
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配列類似性Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
細胞内局在Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
- Information by UniProt
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参照データベース
- Entrez Gene: 3516 Human
- Entrez Gene: 19664 Mouse
- Omim: 147183 Human
- SwissProt: Q06330 Human
- SwissProt: P31266 Mouse
- Unigene: 479396 Human
- Unigene: 209292 Mouse
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別名
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
画像
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Western blot - Anti-RBPJK antibody - ChIP Grade (ab25949)Anti-RBPJK antibody - ChIP Grade (ab25949) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 20 µg
Secondary
Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25949 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.
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Immunoprecipitation - Anti-RBPJK antibody - ChIP Grade (ab25949)RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK
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Western blot - Anti-RBPJK antibody - ChIP Grade (ab25949)This image is courtesy of an Abreview submitted by JK FarmahaAll lanes : Anti-RBPJK antibody - ChIP Grade (ab25949) at 1/500 dilution
Lane 1 : Mouse myoblast C2C12 whole cell lysate at 50 µg
Lane 2 : Mouse myoblast C2C12 whole cell lysate at 75 µg
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minute
プロトコール
参考文献
This product has been referenced in:
- Roese-Koerner B et al. Reciprocal Regulation between Bifunctional miR-9/9(*) and its Transcriptional Modulator Notch in Human Neural Stem Cell Self-Renewal and Differentiation. Stem Cell Reports 7:207-19 (2016). IP . Read more (PubMed: 27426040) »
- Lu F et al. EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-j? and EBF1. PLoS Pathog 12:e1005339 (2016). WB, ChIP . Read more (PubMed: 26752713) »
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