製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RBPJK
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 258-498 of Human RBPJK (NP_005340)

  • ポジティブ・コントロール
    • 293T, A431, HeLa, MOLT4 and Raji cells

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • 配列類似性
    Belongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • 細胞内局在
    Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI843960 antibody
    • AOS3 antibody
    • CBF 1 antibody
    • CBF-1 antibody
    • CBF1 antibody
    • csl antibody
    • IGKJRB antibody
    • IGKJRB1 antibody
    • J kappa recombination signal binding protein antibody
    • J kappa-recombination signal-binding protein antibody
    • KBF2 antibody
    • NY REN 30 antigen antibody
    • RBP J antibody
    • RBP J kappa antibody
    • RBP JK antibody
    • RBP-J antibody
    • RBP-J kappa antibody
    • RBP-JK antibody
    • Rbpj antibody
    • RBPJK antibody
    • RBPSUH antibody
    • recombination signal binding protein for immunoglobulin kappa J region antibody
    • Recombining binding protein suppressor of hairless antibody
    • Renal carcinoma antigen NY-REN-30 antibody
    • SUH antibody
    • SUH_HUMAN antibody
    see all

画像

  • Anti-RBPJK antibody (ab97524) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 56 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97524 at 1/100 dilution. Lower panel is merged with DNA probe.

参考文献

ab97524 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cartilage)
Specification
Cartilage
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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投稿 Jan 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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