製品の概要

  • 製品名Anti-RBP4 antibody
    RBP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RBP4
  • アプリケーション適用あり: WB, ELISA, RIA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
  • 関連疾患Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.
  • 配列類似性Belongs to the calycin superfamily. Lipocalin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • OTTHUMP00000020114 antibody
    • OTTHUMP00000020115 antibody
    • OTTHUMP00000020116 antibody
    • Plasma retinol binding protein 4 antibody
    • Plasma retinol-binding protein antibody
    • Plasma retinol-binding protein(1-176) antibody
    • PRBP antibody
    • PRO2222 antibody
    • RBP antibody
    • RBP4 antibody
    • RDCCAS antibody
    • RET4_HUMAN antibody
    • Retinol binding protein 4 antibody
    • Retinol binding protein 4 interstitial antibody
    • Retinol binding protein 4 plasma antibody
    see all

Anti-RBP4 antibody 画像

  • Ab64194 staining Human normal pituitary gland. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-RBP4 antibody (ab64194) 使用論文

This product has been referenced in:
  • Cukras C  et al. Exome analysis identified a novel mutation in the RBP4 gene in a consanguineous pedigree with retinal dystrophy and developmental abnormalities. PLoS One 7:e50205 (2012). WB ; Human . Read more (PubMed: 23189188) »
  • Holzer M  et al. Uremia Alters HDL Composition and Function. J Am Soc Nephrol 22:1631-41 (2011). WB ; Human . Read more (PubMed: 21804091) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"