製品の概要

  • 製品名Anti-RBM4B antibody
    RBM4B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RBM4B
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: AAAAATTSSY YGRDRSPLRR AAAMLPTVGE GYGYGPESEL SQASAATRNS, corresponding to C terminal amino acids 289-338 of Human RBM4B

  • ポジティブ・コントロール
    • Jurkat cell lysate and human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

Anti-RBM4B antibody 画像

  • Anti-RBM4B antibody (ab51345) at 2.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 40 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Ab51345 (4 µg/ml) staining human RBM4B in human kidney by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) were positively labelled.

Anti-RBM4B antibody (ab51345) 使用論文

ab51345 has not yet been referenced specifically in any publications.

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