製品の概要

  • 製品名
    Anti-RBM35A antibody [27H12]
    RBM35A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [27H12] to RBM35A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Horse, Cow, Dog, Human, Pig, Chimpanzee, Opossum
  • 免疫原

    Full length recombinant Mouse RBM35A with a proprietary tag (NP_918944.2).

  • ポジティブ・コントロール
    • RBM35A transfected 293T cell extract
  • 特記事項
    Store undiluted; dilute only prior to immediate use.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab106585 is an IgG fraction antibody purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered.
  • ポリ/モノ
    モノクローナル
  • クローン名
    27H12
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 76 kDa.

ターゲット情報

Anti-RBM35A antibody [27H12] 画像

  • All lanes : Anti-RBM35A antibody [27H12] (ab106585) at 1/1000 dilution

    Lane 1 : GFP transfected 293T cell extract
    Lane 2 : RBM35A transfected 293T cell extract
    Lane 3 : RBM35B transfected 293T cell extract

    Lysates/proteins at 5 µg per lane.

    Secondary
    anti-mouse ECL at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 76 kDa

プロトコール

Anti-RBM35A antibody [27H12] (ab106585) 使用論文

This product has been referenced in:
  • Warzecha CC  et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 29:3286-300 (2010). Read more (PubMed: 20711167) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, HCT116)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
HEK293, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Simon Boudreault

Verified customer

投稿 Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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