製品の概要

  • 製品名
    Anti-RBM26 antibody
    RBM26 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RBM26
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • 免疫原

    Synthetic peptide corresponding to a region between residues 450 and 500 of human RBM26 (NP_071401.3)

  • ポジティブ･コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    ab74198 was affinity purified using an epitope specific to RBM26 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性
    RBM26 (RNA binding motif protein 26) bears two RNA recognition motif (RRM) domains and a C3H1-type zinc finger. The function remains unknown.
  • 参照データベース
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    画像

    • Western blot - Anti-RBM26 antibody (ab74198)
      Western blot - Anti-RBM26 antibody (ab74198)
      All lanes : Anti-RBM26 antibody (ab74198) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 114 kDa
      Observed band size: 125 kDa (why is the actual band size different from the predicted?)


      Exposure time: 5 seconds
    • Immunoprecipitation - Anti-RBM26 antibody (ab74198)
      Immunoprecipitation - Anti-RBM26 antibody (ab74198)
      Detection of RBM26 by Western Blot of Immunprecipitate.
      ab74198, at 1µg/ml, staining RBM26 in HeLa whole cell lysate immunoprecipitated using ab74198 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 1 second.

    参考文献

    ab74198 has not yet been referenced specifically in any publications.

    ab74198 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1 Review or Q&A

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    Western blot abreview for Anti-RBM26 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NSC34 cells)
    Loading amount
    10 µg
    Specification
    NSC34 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Jul 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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