製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RBM25
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
  • 免疫原

    Synthetic peptide corresponding to a region between residue 475 and 525 of human RBM25 (120 kDa) AAF19255.1

  • ポジティブ・コントロール
    • Human Colon Carcinoma, Prostate Carcinoma, Mouse Teratoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA.
    • 配列類似性
      Contains 1 PWI domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻訳後修飾
      Sumoylated.
    • 細胞内局在
      Nucleus speckle. Cytoplasm. Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Arg/Glu/Asp rich protein, 120 kDa antibody
      • Arg/Glu/Asp-rich protein of 120 kDa antibody
      • fSAP94 antibody
      • functional spliceosome-associated protein 94 antibody
      • NET52 antibody
      • Protein S164 antibody
      • Rbm25 antibody
      • RBM25_HUMAN antibody
      • RED120 antibody
      • RNA binding motif protein 25 antibody
      • RNA binding region (RNP1, RRM) containing 7 antibody
      • RNA-binding motif protein 25 antibody
      • RNA-binding protein 25 antibody
      • RNA-binding region-containing protein 7 antibody
      • RNPC7 antibody
      • S164 antibody
      • Snu71 antibody
      • U1 small nuclear ribonucleoprotein 1SNRP homolog antibody
      see all

    画像

    • Detection of Human RBM25 by ab84460 at 1:500 dilution using a Formalin-Fixed Paraffin-Embedded section of human prostate adenocarcinoma. Detection by means of DAB staining.
    • Detection of Human RBM25 by ab84460 at 1:100 dilution using a Formalin-Fixed Paraffin-Embedded section of human colon carcinoma. Detection by means of DAB staining.
    • Detection of Mouse RBM25 by ab84460 at 1:100 dilution using a Formalin-Fixed Paraffin-Embedded section of mouse teratoma. Detection by means of DAB staining.

    プロトコール

    参考文献

    ab84460 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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