製品の概要

  • 製品名Anti-RBM25 antibody
    RBM25 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RBM25
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide from a region between residue 475 and 525 of human RBM25

  • ポジティブ・コントロール
    • Whole cell lysate from Hela cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 71, 117 kDa (predicted molecular weight: 94 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA.
  • 配列類似性Contains 1 PWI domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Nucleus speckle. Cytoplasm. Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arg/Glu/Asp rich protein, 120 kDa antibody
    • Arg/Glu/Asp-rich protein of 120 kDa antibody
    • fSAP94 antibody
    • functional spliceosome-associated protein 94 antibody
    • NET52 antibody
    • Protein S164 antibody
    • Rbm25 antibody
    • RBM25_HUMAN antibody
    • RED120 antibody
    • RNA binding motif protein 25 antibody
    • RNA binding region (RNP1, RRM) containing 7 antibody
    • RNA-binding motif protein 25 antibody
    • RNA-binding protein 25 antibody
    • RNA-binding region-containing protein 7 antibody
    • RNPC7 antibody
    • S164 antibody
    • Snu71 antibody
    • U1 small nuclear ribonucleoprotein 1SNRP homolog antibody
    see all

Anti-RBM25 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RBM25 with ab72237 at 1/200 (1 µg/ml). Detection: DAB.
  • All lanes : Anti-RBM25 antibody (ab72237) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg


    Predicted band size : 94 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
    Various other bands are detected.
  • Immunoprecipitation/ Western Blot of RBM25.
    Lane 1: ab72237 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72237 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.

Anti-RBM25 antibody (ab72237) 使用論文

ab72237 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (hESC)
Gel Running Conditions Non-reduced Denaturing (4-20%)
Loading amount 20 µg
Specification hESC
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Miguel Fidalgo

Verified customer

投稿 Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"