Anti-RBM25 抗体 (ab72237)
Key features and details
- Rabbit polyclonal to RBM25
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBM25 antibody -
製品の詳細
Rabbit polyclonal to RBM25 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus -
免疫原
Synthetic peptide from a region between residue 475 and 525 of human RBM25
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ポジティブ・コントロール
- Whole cell lysate from Hela cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibody was affinity purified using an epitope specific to BTAF1 immobilized on solid support -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72237の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 71, 117 kDa (predicted molecular weight: 94 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 71, 117 kDa (predicted molecular weight: 94 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. -
配列類似性
Contains 1 PWI domain.
Contains 1 RRM (RNA recognition motif) domain. -
翻訳後修飾
Sumoylated. -
細胞内局在
Nucleus speckle. Cytoplasm. Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint. - Information by UniProt
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参照データベース
- Entrez Gene: 58517 Human
- Entrez Gene: 67039 Mouse
- Entrez Gene: 366693 Rat
- Omim: 612427 Human
- SwissProt: P49756 Human
- SwissProt: B2RY56 Mouse
- Unigene: 531106 Human
- Unigene: 46005 Mouse
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別名
- Arg/Glu/Asp rich protein, 120 kDa antibody
- Arg/Glu/Asp-rich protein of 120 kDa antibody
- fSAP94 antibody
see all
画像
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All lanes : Anti-RBM25 antibody (ab72237) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 94 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.
Various other bands are detected. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RBM25 with ab72237 at 1/200 (1 µg/ml). Detection: DAB.
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Immunoprecipitation/ Western Blot of RBM25.
Lane 1: ab72237 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72237 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab72237 は 4 報の論文で使用されています。
- Kundinger SR et al. Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization. J Biol Chem 297:101306 (2021). PubMed: 34673031
- Schleicher EM et al. Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. PLoS Genet 16:e1009176 (2020). PubMed: 33137164
- Velazquez R et al. Maternal choline supplementation ameliorates Alzheimer's disease pathology by reducing brain homocysteine levels across multiple generations. Mol Psychiatry N/A:N/A (2019). PubMed: 30622336
- Bishof I et al. RNA-binding proteins with basic-acidic dipeptide (BAD) domains self-assemble and aggregate in Alzheimer's disease. J Biol Chem 293:11047-11066 (2018). PubMed: 29802200