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Synthetic peptide (Human). Amino acid regions from which the immunogen sequence was derived, KWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYF .
Our Abpromise guarantee covers the use of ab59157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay:1/1562500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling RBM22 with ab59157 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling RMB22 with ab59157 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.
ab59157 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"