製品の概要

  • 製品名Anti-RBM17 antibody
    RBM17 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RBM17
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 227 and 370 of Human RBM17 (AAH07871).

  • ポジティブ・コントロール
    • 293T and Raji cell lines

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.

ターゲット情報

  • 機能Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
  • 配列類似性Contains 1 G-patch domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 45 kDa-splicing factor antibody
    • RBM 17 antibody
    • Rbm17 antibody
    • RNA binding motif protein 17 antibody
    • RNA-binding motif protein 17 antibody
    • SPF 45 antibody
    • SPF45 antibody
    • SPF45_HUMAN antibody
    • Splicing factor (45 kD) antibody
    • Splicing factor (45kD) antibody
    • Splicing factor 45 antibody
    • Splicing factor 45 kDa antibody
    • Splicing factor 45kDa antibody
    see all

Anti-RBM17 antibody 画像

  • Anti-RBM17 antibody (ab101441) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 45 kDa

Anti-RBM17 antibody (ab101441) 使用論文

ab101441 has not yet been referenced specifically in any publications.

Product Wall

The antibody has not been tested on mouse samples before, so there may be an issue with low cross-reactivity with mouse RBM17, though reactivity is predicted based on homology with the immunogen. In any case, the high background is the main issue.
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In my opinion, the 54 kDa band is phosphorylated RBM17 and the lower 45 kDa band is the dephosphorylated version. The 70 kDa band appears to be a non-specific band.

I came to these conclusions looking at the western blots for two other an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"