製品の概要

  • 製品名Anti-RBM14 antibody
    RBM14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RBM14
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 550 and 600 of Human RBM14 (NP_006319.1)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).
IP Use at 2 µg/mg of lysate.

ターゲット情報

  • 関連性RBM14 is a RNA-binding protein. These proteins contribute to gene expression by regulating the form, abundance and stability of both coding and non-coding RNAs. In vertebrates, RNA binding proteins can also influence many processes involved in RNA processing, for example, activity-dependent transcript localization and localized protein synthesis amongst others.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • CoAA antibody
    • Coactivator Activator antibody
    • RBM 14 antibody
    • RBM14 antibody
    • RNA binding motif protein 14 antibody
    • RNA binding protein 14 antibody
    • RRM containing coactivator activator/modulator antibody
    • SIP antibody
    • Synaptotagmin interacting protein antibody
    • SYT interacting protein 1 antibody
    • SYT interacting protein antibody
    • SYT interacting protein SIP antibody
    • SYTIP 1 antibody
    • SYTIP1 antibody
    see all

Anti-RBM14 antibody 画像

  • All lanes : Anti-RBM14 antibody (ab70636) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of RBM14 by Western Blot of Immunprecipitate. ab70636 at 1µg/ml staining RBM14 in HeLa whole cell lysate immunoprecipitated using ab70636 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
  • ICC/IF image of ab70636 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70636, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70636 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70636, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-RBM14 antibody (ab70636) 使用論文

ab70636 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C antibody (sc-15386) bound proetin A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads
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投稿 Jun 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"