製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RbBP5
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RbBP5.

    (Peptide available as ab87828.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能
    As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Contains 6 WD repeats.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • RBBP 5 antibody
    • RBBP-5 antibody
    • RBBP5 antibody
    • RBBP5_HUMAN antibody
    • RBQ 3 antibody
    • RBQ3 antibody
    • Retinoblastoma binding protein 5 antibody
    • Retinoblastoma binding protein RBQ3 antibody
    • Retinoblastoma binding protein5 antibody
    • Retinoblastoma-binding protein 5 antibody
    • Retinoblastoma-binding protein RBQ-3 antibody
    • SWD1 antibody
    • SWD1, Set1c WD40 repeat protein, homolog antibody
    see all

画像

  • All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml

    Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 60,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

参考文献

ab80074 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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