製品の概要

  • 製品名Anti-RbBP5 antibody
    RbBP5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RbBP5
  • アプリケーション適用あり: WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide mapping to a region between a.a 500 and C-terminus (538) of human RbBP5.

  • ポジティブ・コントロール
    • 293T cell lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Contains 6 WD repeats.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • RBBP 5 antibody
    • RBBP-5 antibody
    • RBBP5 antibody
    • RBBP5_HUMAN antibody
    • RBQ 3 antibody
    • RBQ3 antibody
    • Retinoblastoma binding protein 5 antibody
    • Retinoblastoma binding protein RBQ3 antibody
    • Retinoblastoma binding protein5 antibody
    • Retinoblastoma-binding protein 5 antibody
    • Retinoblastoma-binding protein RBQ-3 antibody
    • SWD1 antibody
    • SWD1, Set1c WD40 repeat protein, homolog antibody
    see all

Anti-RbBP5 antibody 画像

  • ICC/IF image of ab52084 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RbBP5 antibody (ab52084) 使用論文

This product has been referenced in:
  • Benedikt A  et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB ; Human . Read more (PubMed: 21030982) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 40 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"