製品の概要
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製品名Anti-RbAp48 antibody
RbAp48 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to RbAp48
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human p48 CAF1.
(Peptide available as ab61624.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following Lysates: HeLa Whole Cell Lysate Rat Lung Tissue Human Lung Tissue Mouse Spleen Tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab47456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 0.1 - 1 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
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配列類似性Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 395658 Chicken
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Entrez Gene: 100461561 Orangutan
- Entrez Gene: 313048 Rat
- Omim: 602923 Human
- SwissProt: Q9W7I5 Chicken
- SwissProt: Q09028 Human
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別名
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RbAp48 antibody (ab47456)IHC image of RbAp48 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-RbAp48 antibody (ab47456)This image is courtesy of an anonymous AbreviewAll lanes : Anti-RbAp48 antibody (ab47456) at 1/2500 dilution
Lane 1 : Mouse MEL (erythroleukemia) cell lysate
Lane 2 : Human K562 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 1 minute
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Western blot - Anti-RbAp48 antibody (ab47456)All lanes : Anti-RbAp48 antibody (ab47456) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 : Human lung normal tissue lysate
Lane 4 :Mouse spleen tissue lysate - total protein (ab29293)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-RbAp48 antibody (ab47456)ICC/IF image of ab47456 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47456, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% pfa fixed (10 min) HepG2, MCF-7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2, MCF-7 cells at 1µg/ml.
プロトコール
参考文献
This product has been referenced in:
- Yang C et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). Read more (PubMed: 27794043) »
レビューと Q&A
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