製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RbAp48
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Dog, Human
    交差が予測される動物種: Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    CENIYNDEDPEGSVDPEGQGS

    , corresponding to amino acids 406-425 of Human and Mouse CAF 1 (p48).

  • 特記事項
    Locus link: 350005 Alternative names: similar to chromatin assembly factor 1 p48 subunit


    A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 20 µg/ml.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa).

ターゲット情報

  • 機能
    Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • 配列類似性
    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAF I p48 antibody
    • CAF-1 subunit C antibody
    • CAF-I 48 kDa subunit antibody
    • CAF-I p48 antibody
    • Chromatin assembly factor 1 subunit C antibody
    • Chromatin assembly factor I p48 subunit antibody
    • Chromatin assembly factor/CAF 1 p48 subunit antibody
    • Histone-binding protein RBBP4 antibody
    • MSI1 protein homolog antibody
    • Nucleosome-remodeling factor subunit RBAP48 antibody
    • NURF55 antibody
    • RbAp 48 antibody
    • RBAP48 antibody
    • RBBP-4 antibody
    • RBBP4 antibody
    • RBBP4_HUMAN antibody
    • Retinoblastoma binding protein 4 antibody
    • Retinoblastoma binding protein p48 antibody
    • Retinoblastoma-binding protein 4 antibody
    • Retinoblastoma-binding protein p48 antibody
    see all

Anti-RbAp48 antibody 画像

  • ab1765 staining RbAp48 in MDCK cell pellets by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Pellets were fixed with paraformaldehyde and blocked with 1% BSA for 3 hours at 22°C; antigen retrieval was by heat mediation in a citrate buffer, permeabilization was with Tween-20. Samples were incubated with primary antibody (1/100 in TBST) for 16 hours. A HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview



  • Predicted band size : 47.6 kDa

    Rabbit polyclonal to CAF 1 p48 (ab1765)
    Each lane contains 20 ug of HeLa whole cell lysate.

    Lane 1: ab1765 1/500
    Lane 2: ab1765 1/1000

    Secondary antibody - Goat anti-Rabbit (HRP) (ab6721) at 1/2000.

  • Anti-RbAp48 antibody (ab1765) at 1/1000 dilution

    Secondary
    Anti-rabbit IgG HRP
    Developed using the ECL technique

    Predicted band size : 47.6 kDa
    Observed band size : 40,55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minuteab1765 detecting p48 CAF1 from human LCL whole cell lysates by Western Blot.

    See Abreview

  • ab1765 used in Immunoprecipitation of HeLa whole cell lysate.

    Whole cell extract (containing 100µg of soluble protein) was incubated with 20µg of antibody overnight. Protein A-Agarose was added to the immunocomplexes and incubated at room temperature for three hours. Immunocomplexes were eluted and then resolved using 7.5% SDS-PAGE under reducing conditions. For the detection of HDAC1, 2 and 3 a non-Abcam mouse monoclonal antibody was used at a dilution of 1/800. Results support the understanding that HDAC1 and 2 are in the same complex together with p48. Note that HDAC1 does not coprecipitate HDAC2, probably displaced by the antibody during the experiment.

    Lane 1: Input

    Lane 2: HDAC1 (ab1769)

    Lane 3: p48 (ab1765)

    Lane 4: No antibody control

    This image is courtesy of an Abreview by Hugh Spotswood submitted on 9 February 2006.

    See Abreview

  • ab1765 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

Anti-RbAp48 antibody (ab1765) 使用論文

This product has been referenced in:
  • Tian LQ  et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). Read more (PubMed: 27035789) »
  • Müller S  et al. Phosphorylation and DNA binding of HJURP determine its centromeric recruitment and function in CenH3(CENP-A) loading. Cell Rep 8:190-203 (2014). WB . Read more (PubMed: 25001279) »

See all 5 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample
Human Tissue sections (Human fibromatosis)
Specification
Human fibromatosis
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Username

Abcam user community

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投稿 Dec 05 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - Triton 1%
Fixative
Paraformaldehyde
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投稿 Dec 02 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Dog Tissue sections (MDCK pellets in paraffin)
Specification
MDCK pellets in paraffin
Permeabilization
Yes - Tween-20
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Alexander Rapp

Verified customer

投稿 Apr 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Human HeLa cell lysate)
Specification
Human HeLa cell lysate
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

投稿 May 15 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES cells)
Specification
Mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 20 2007

This product has been tested in IF and would be gaurateed to work in that application.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
25 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 12 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LCL)
Specification
LCL
Blocking step
Other as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. Hannes Meyer

Verified customer

投稿 Dec 12 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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