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Fusion protein containing the complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation.
Our Abpromise guarantee covers the use of ab488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47.5 kDa.Can be blocked with RbAp48 peptide (ab5014).
Dilute in 5% Milk/PBST and incubate at 4ºC overnight. Use under reducing conditions.
|IP||Use a concentration of 1 - 5 µg/ml.|
|ICC/IF||1/500 - 1/1000.
Permeabilization step recommended. Use in PBST containing 2% BSA and 10% normal serum at 4ºC overnight.
This image is courtesy of an anonymous Abreview
Image from S Maenner et al, PLoS Biol 8:e1000276 (2010), Fig 9.