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RabMAb

Anti-Rb2 p130 (phospho S952) 抗体 [EP2387AY] (ab68136)

製品の概要

  • 製品名
    Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]
    Rb2 p130 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952)
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding serine 952 of human p130

  • ポジティブ・コントロール
    • Jurkat cell lysate; human breast carcinoma tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab68136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
  • 追加情報
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250.
    WB: 1/10000 - 1/500000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
    • 細胞内局在
      Nuclear
    • 参照データベース
    • 別名
      • 130 kDa retinoblastoma associated protein antibody
      • FLJ26459 antibody
      • p130 antibody
      • PRB 2 antibody
      • PRB2 antibody
      • Rb 2 antibody
      • Rb2 antibody
      • RBL 2 antibody
      • RBL2 antibody
      • RBR 2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma like protein 2 antibody
      • Retinoblastoma related gene antibody
      see all

    画像

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68136 at a dilution of 1/100.
    • All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/500000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-mouse at 1/2000 dilution

      Predicted band size : 128 kDa
      Observed band size : 128 kDa

    参考文献

    This product has been referenced in:
    • Divakar SK  et al. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia 30:86-93 (2016). Read more (PubMed: 26174628) »
    • Orlando S  et al. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res 43:6860-73 (2015). Read more (PubMed: 26071952) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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