Anti-Rb2 p130 (phospho S952) 抗体 [EP2387AY] (ab68136)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]
Rb2 p130 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat and RAW 264.7 cell lysate; mouse heart tissue lysate IHC-P: human breast and lung carcinoma tissue, Mouse cerebrum
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2387AY -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68136の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
For unpurified use at 1/10000 - 1/500000. |
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IHC-P |
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
特記事項 |
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WB
1/2000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa). For unpurified use at 1/10000 - 1/500000. |
IHC-P
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
ターゲット情報
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関連性
Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 5934 Human
- Entrez Gene: 19651 Mouse
- Omim: 180203 Human
- SwissProt: Q08999 Human
- SwissProt: 235580 Mouse
- SwissProt: Q64700 Mouse
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別名
- 130 kDa retinoblastoma associated protein antibody
- FLJ26459 antibody
- p130 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa -
All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/5000 dilution (Purified)
Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates. Then the membrane was incubated with phosphatase
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa -
All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution (Purified)
Lane 1 : Mouse heart lysates. Then the membrane was incubated with phosphatase
Lane 2 : Mouse heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/500000 dilution (unpurified)
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-mouse at 1/2000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68136 (unpurified) at a dilution of 1/100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab68136 は 3 報の論文で使用されています。
- Su M et al. Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication. J Virol 95:e0018721 (2021). PubMed: 34037422
- Divakar SK et al. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia 30:86-93 (2016). PubMed: 26174628
- Orlando S et al. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res 43:6860-73 (2015). PubMed: 26071952