製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Rb2 p130
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    KYPQEEQPRQQRGRK

    , corresponding to amino acids 175-189 of Human Rb2 p130

  • ポジティブ・コントロール
    • HeLa and Jurkat T cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

ターゲット情報

  • 関連性
    Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • 130 kDa retinoblastoma associated protein antibody
    • FLJ26459 antibody
    • p130 antibody
    • PRB 2 antibody
    • PRB2 antibody
    • Rb 2 antibody
    • Rb2 antibody
    • RBL 2 antibody
    • RBL2 antibody
    • RBR 2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma like protein 2 antibody
    • Retinoblastoma related gene antibody
    see all

画像

  • All lanes : Anti-Rb2 p130 antibody (ab71143) at 1/2000 dilution

    Lane 1 : Jurkat T cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 128 kDa
    Observed band size : 128 kDa

プロトコール

参考文献

ab71143 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19 cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Specification
ARPE-19 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 18 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Vero cell)
Permeabilization
Yes - 0.25% Tx-100
Specification
Vero cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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