製品の概要

  • 製品名Anti-Rb (phospho T821) antibody
    Rb 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rb (phospho T821)
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (Human) derived from a region of human Rb that contains threonine 821 (based on Swiss Protein database, accession number P06400). The sequence is conserved in human, mouse and rat.

  • ポジティブ・コントロール
    • Jurkat cells in high growth phase.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab4787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 106 kDa).
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
  • 組織特異性Expressed in the retina.
  • 関連疾患Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma
  • 配列類似性Belongs to the retinoblastoma protein (RB) family.
  • ドメインThe Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
  • 翻訳後修飾Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • OSRC antibody
    • Osteosarcoma antibody
    • p105-Rb antibody
    • P105RB antibody
    • PP105 antibody
    • pp110 antibody
    • PPP1R130 antibody
    • pRb antibody
    • Prepro retinoblastoma associated protein antibody
    • Protein phosphatase 1 regulatory subunit 130 antibody
    • Rb antibody
    • RB_HUMAN antibody
    • RB1 antibody
    • RB1 gene antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma suspectibility protein antibody
    • Retinoblastoma-associated protein antibody
    see all

Anti-Rb (phospho T821) antibody 画像



  • Predicted band size : 106 kDa
    Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4787, following prior incubation in the: absence of the phosphopeptide immunogen (1), a generic phosphothreonine containing peptide (2), the non phosphopeptide corresponding to the phosphopeptide immunogen (3), or the presence of the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab4787 antibody for this epitope. Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were in
  • ab4787 staining Rb (phospho T821) in mouse B cell line cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 3% BSA + 2.5% serum in TBS for 2 hours at room temperature. Samples were incubated with primary antibody (1/300 in TBS-T + 1% serum + 3% BSA) for 16 hours at 4°C. An Alexa Fluor® 546-conjugated goat anti-rabbit IgG (H+L) polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-Rb (phospho T821) antibody (ab4787) 使用論文

This product has been referenced in:
  • Labalette C  et al. The LIM-only Protein FHL2 Regulates Cyclin D1 Expression and Cell Proliferation. J Biol Chem 283:15201-15208 (2008). WB ; Mouse . Read more (PubMed: 18378678) »
  • Panic L  et al. Ribosomal protein S6 gene haploinsufficiency is associated with activation of a p53-dependent checkpoint during gastrulation. Mol Cell Biol 26:8880-91 (2006). WB ; Mouse . Read more (PubMed: 17000767) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Sample Mouse Cell (B cell line)
Specification B cell line
Permeabilization Yes - 0,1% TritonX-100/PBS for 10 minutes at RT
Fixative Paraformaldehyde
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投稿 Jun 03 2014

Thank you for contacting us. We currently only have the tagged Rb proteins in our catalog and no blocking peptide for ab4787 is available. I would sugges Biocompare.com to search for these products. I hope this information is helpful to you. Please ...

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