製品の概要

  • 製品名Rat TNF alpha ELISA Set (without plates)
    TNF alpha キット 製品一覧
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    31.25 pg/ml - 1000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s TNFα Rat in vitro ELISA Set is designed for the quantitative measurement of Rat TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant Rat TNF alpha Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームReagents

製品の特性

  • 関連性TNF-alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF-alpha is a potent pyrogen causing fever by direct action or by stimulation of interleukin 1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 細胞内局在Cell membrane; single-pass type II membrane protein. Processed form: Secreted protein.
  • 別名
    • Cachectin
    • TNF
    • TNF alpha
    • TNFA
    • TNFSF2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab48419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Rat TNF alpha ELISA Set (without plates) 画像

    Rat TNF alpha ELISA Set (without plates) (ab48419) 使用論文

    ab48419 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"