• 製品名
    Rat TNF alpha ELISA Kit
    TNF alpha キット 製品一覧
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts, Tissue Extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 25 pg/ml
  • 検出範囲
    82.3 pg/ml - 20000 pg/ml
  • 添加回収試験

    92 %

    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 93.17 81% - 105%
    Tissue Extracts 92.48 80% - 104%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s TNF alpha ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in cell lysates and tissue lysates.

    This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    2x Cell lysate buffer 1 x 5ml
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-Rat TNF alpha  (lyophilized)  2 vials
    Recombinant Rat TNF alpha Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TNF alpha Microplate (12 x 8 wells) 1 unit
  • 研究分野
  • 機能
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Rat TNF alpha detected in cell lysates from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma). Results shown for TNF alpha levels in 10e6 cells after background signal was subtracted (duplicates +/- SD).

  • Rat TNF alpha detected in cell supernatants from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma); background signal subtracted (duplicates +/- SD).

  • Representative standard curve using ab100785



This product has been referenced in:
  • Jensen VS  et al. Dietary fat stimulates development of NAFLD more potently than dietary fructose in Sprague-Dawley rats. Diabetol Metab Syndr 10:4 (2018). Read more (PubMed: 29410708) »
  • Margonis GA  et al. Effectiveness of sildenafil and U-74389G in a rat model of colitis. J Surg Res 193:667-74 (2015). Read more (PubMed: 25277360) »

See all 4 Publications for this product

レビューと Q&A

Yes, that lysis buffer will be good, the 150 mM sodium chloride, 1.0% Triton X-100, 50 mM Tris pH 8.0.

Here are some general guidelines.

Avoid using >0.1% SDS or other strongly denaturing detergents. In general, non-ionic de...

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Thank you for contacting us. The cell lysis buffer does not contain any protease inhibitor. Please let me know if you have any further questions.

I can suggest the following general tissue lysate preparation procedure to prepare samples for both the kits.

Tissue lyste samples can be prepared using most conventional methods, for example with RIPA or other formula...

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Thank you for your enquiry.

Please rest assured that there are no hazardous components in this kit according to Japanese law, and is safe for use by customers in Japan.

Have a nice day!

Abreview for TNF alpha Rat ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Rat Tissue lysate - whole (spinal cord)
Specification: spinal cord
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Traumatized segment level T13
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: I have used the kit with fresh rat spinal cord samples that underwent compression. The samples where homogenized a day before and kept in -20c. The strips allowed me to use the kit 3 times. The third time was a week after I have open Item F and still got a nice curve. R-square=0.9959.
Results are more than satisfying. It is a quick and straight forward assay.

Graph labels: Y axis is absorbance, X axis is pg/ml.The key labels are, from top to bottom: Standards, suppresses stds, standard curve.

Prof. Guy Sovak

Verified customer

投稿 May 21 2012

Thank you for your enquiry.

The lysis buffer (item J) is used to prepare the cell and tissue sample. There is no mention on how to prepare your sample in the Protocol Booklet as most laboratories would have their own protocols and methods fo...

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