製品の概要

  • 製品名
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 300 pg/ml
  • 検出範囲
    0.41 ng/ml - 300 ng/ml
  • 添加回収試験

    72 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Plasma 74.84 69% - 88%
    Cell culture media 64.74 53% - 70%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s RAGE Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat RAGE in plasma and cell culture supernatants.


    This assay employs an antibody specific for Rat RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat RAGE in normal serum with ab100780, only in serum spiked with Rat RAGE.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100780

プロトコール

参考文献

This product has been referenced in:
  • Hurtgen BJ  et al. Autologous minced muscle grafts improve endogenous fracture healing and muscle strength after musculoskeletal trauma. Physiol Rep 5:N/A (2017). Functional Studies ; Rat . Read more (PubMed: 28747511) »

See 1 Publication for this product

レビューと Q&A

The laboratory has informed me that ab100780 will detect soluble RAGE.

Thank you for contacting us.


Although this particular kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test th...

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Thank you for calling Abcam yesterday.

I have talked to the lab and we should be able to add the standard from ab100780 to the catalogue. It may take a week or so though. I will contact you with the abID when it is ready to be ordered.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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