製品の概要

  • 製品名Rat Prolactin R ELISA Kit (PRLR)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.4 ng/ml
  • 検出範囲
    0.512 ng/ml - 50 ng/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 125.4 117% - 141%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s Prolactin R Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Prolactin R in cell culture supernatants.

    This assay employs an antibody specific for rat Prolactin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Prolactin R antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Note: very low signal were obtained in normal serum and plasma

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • 組織特異性Expressed in breast, placenta, kidney, liver and pancreas.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 細胞内局在Secreted and Membrane.
  • Information by UniProt
  • 別名
    • PRL-R
    • PRLR
    • PRLR_HUMAN
    • Prolactin receptor
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat Prolactin R ELISA Kit (PRLR) 画像

  • Representative standard curve using ab113351

プロトコール

Rat Prolactin R ELISA Kit (PRLR) (ab113351) 使用論文

This product has been referenced in:
  • Adedara IA  et al. Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Arch Environ Contam Toxicol N/A:N/A (2014). Read more (PubMed: 25179371) »

See 1 Publication for this product

Product Wall

Thank you for your interest in our Prolactin R Rat ELISA, ab113351. The laboratory that developed the ELISA has not tested it on milk or pituitary samples, but does not expect that there will be any problems. We recommend following the kit protocol sug...

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The laboratory has confirmed that this kit will not detect mouse Prolactin R. The rabbit polyclonal capture antibody will, but not the mouse monoclonal detection antibody.

Gracias por contactarnos.


El anticuerpo usado como captura cubriendo la placa procede de cabra, y es policlonal. El otro anticuerpo, el de detección se ha producido en ratón, y es monoclonal.

Espero haberte ayudad...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"