製品の概要

  • 製品名Rat MCP1 ELISA Kit (CCL2)
    MCP1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    24.67 pg/ml - 18000 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.4 86% - 105%
    Serum 95.39 84% - 103%
    Plasma 92.44 83% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 2
    • CCL2
    • CCL2_HUMAN
    • Chemokine (C C motif) ligand 2
    • GDCF 2
    • GDCF-2
    • GDCF2
    • HC11
    • HSMCR30
    • JE
    • MCAF
    • MCP 1
    • MCP-1
    • MCP1
    • MGC9434
    • Monocyte chemoattractant protein 1
    • Monocyte chemotactic and activating factor
    • Monocyte chemotactic protein 1
    • Monocyte secretory protein JE
    • SCYA2
    • Small inducible cytokine A2
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je)
    • Small inducible cytokine subfamily A (Cys Cys), member 2
    • Small-inducible cytokine A2
    • SMC CF
    • SMC-CF
    • SMCCF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat MCP1 ELISA Kit (CCL2) 画像

  • Representative standard curve using ab100777

  • Representative standard curve using ab100777

プロトコール

Rat MCP1 ELISA Kit (CCL2) (ab100777) 使用論文

ab100777 has not yet been referenced specifically in any publications.

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I am sorry to hear that this kit was not supplied to you in full.

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I h...

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Thank you for your enquiry.

I have addressed your questions below.
1. Firstly I need to use the kit to detect urinary MCP-1, so which Diluent should I use: Diluent A or B?

Assay Diluent A is optimized specifically for only se...

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Thank you for contacting us.

Although we have not tested and validated this ELISA kit with urine samples, based on previous testing with our ELISA kits the Rat MCP1 ELISA is very likely to work for urine.

Some general guidelines on ...

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Thank you for your enquiry. I apologize for the delay in replying.

The two kits use the same antibodies but a few other reagents in each kit differ, to optimize the assay for different sample types. Product ab100777 is best for plasma, serum...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"