Rat liver tissue lysate - mitochondrial extract (ab110346)
製品の概要
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製品名
Rat liver tissue lysate - mitochondrial extract
Rat Liver ライゼート 製品一覧 -
特記事項
Product was previously marketed under the MitoSciences sub-brand.
2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
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アプリケーション
適用あり: IP, SDS-PAGE, WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
バッファー
pH: 7.5
Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose -
Concentration information loading...
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ライゼート 備考
2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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SDS-PAGE |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
SDS-PAGE
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110346 は論文での使用が確認できていません。