製品の概要

  • 製品名Rat Leptin ELISA Kit
    Leptin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 30 pg/ml
  • 検出範囲
    10.97 pg/ml - 8000 pg/ml
  • 添加回収試験

    84 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 75.43 68% - 89%
    Serum 92.32 83% - 102%
    Plasma 84.25 75% - 94%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 関連疾患Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 配列類似性Belongs to the leptin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • FLJ94114
    • LEP
    • LEP_HUMAN
    • LEPD
    • Leptin
    • Leptin (murine obesity homolog)
    • Leptin (obesity homolog, mouse)
    • Leptin Murine Obesity Homolog
    • Leptin Precursor Obesity Factor
    • OB
    • Obese protein
    • Obese, mouse, homolog of
    • Obesity
    • Obesity factor
    • Obesity homolog mouse
    • Obesity Murine Homolog Leptin
    • OBS
    • OTTHUMP00000212285
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat Leptin ELISA Kit 画像

  • Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample

  • Rat Leptin measured in biofluids showing quantity (ng) per mL of tested sample

  • Standard curve: mean of duplicates (+/-SD) with background readings subtracted

プロトコール

Rat Leptin ELISA Kit (ab100773) 使用論文

ab100773 has not yet been referenced specifically in any publications.

Product Wall

Abreview for Leptin Rat ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Rat Serum (Blood)
Specification: Blood
Amount of Sample: 50 µg
Type of Kit Used: ELISA
Positive control: rat serum (diabetic)
Did you use the Abcam Kit protocol?: Yes
Username

Dr. Asmat Salim

Verified customer

投稿 May 07 2012

Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"