Rat IL-4 ELISA Set (without plates) (ab48423)
製品の概要
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製品名
Rat IL-4 ELISA Set (without plates) -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 15 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s IL-4 Rat in vitro ELISA Set is designed for the quantitative measurement of Rat IL-4 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The rat IL-4 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure rat IL-4 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Rat IL-4 Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 15 x 96 tests 10 x 96 tests 5 x 96 tests Chromogen TMB Substrate Solution 6 vials 4 vials 2 vials Detection biotinylated anti-Rat IL-4 antibody 3 x 0.55ml 2 x 0.55ml 1 x 0.55ml Rat IL-4 Capture antibody 3 x 0.5ml 2 x 0.5ml 1 x 0.5ml Rat IL-4 Standard (Lyophilized) Yellow 15 vials 10 vials 5 vials Streptavidin-HRP 3 vials 2 vials 1 vial -
研究分野
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関連性
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. -
細胞内局在
Secreted -
別名
- B cell stimulatory factor 1
- Binetrakin
- BSF1
see all -
参照データベース
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48423の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
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