Rat IL-6 ELISA Kit (Interleukin-6) (ab119548)

製品の概要

  • 製品名
    Rat IL-6 ELISA Kit (Interleukin-6)
    IL6 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 < 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6 < 10%
  • サンプルの種類
    Cell culture supernatant, Serum
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    12 pg/ml
  • 検出範囲
    31 pg/ml - 2000 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-6 (Interleukin-6) rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-6 in culture supernatant and serum.

    Il-6 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Il-6 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic an stop solution. The density of yellow coloration is directly proportional to the amount of Il-6 captured on the plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-rat IL6 monoclonal antibody 1 x 70µl
    IL-6 Standard lyophilized (4 ng/mL upon reconstitution) 2 vials
    Microplate coated with monoclonal antibody to rat IL-6 (12 x 8 wells) 1 unit
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 機能
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 関連疾患
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 配列類似性
    Belongs to the IL-6 superfamily.
  • 翻訳後修飾
    N- and O-glycosylated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • B cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta-2
    • Interleukin 6
    • Interleukin BSF2
    • Interleukin-6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119548.

プロトコール

参考文献

ab119548 has not yet been referenced specifically in any publications.

レビューと Q&A


I have the following information for you regarding the kits we discussed:


ab46073:
We have no data regarding normal values observed with IL-6 and IL-4 in rat but as free circulating cytokines are rather dangerous for living ani...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録