Rat IL-2 ELISA Kit (ab100769)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.12 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-2 ELISA Kit
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.1 ng/ml -
検出範囲
0.12 ng/ml - 30 ng/ml -
添加回収試験
85 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 78.52 67% - 89% Plasma 80.35 70% - 91% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s IL-2 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in plasma and cell culture supernatants. (Rat IL-2 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat IL-2 in normal serum with ab100769, only in serum spiked with Rat IL-2.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Rat IL-2 2 vials IL-2 Microplate (12 x 8 wells) 1 unit Recombinant rat IL-2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 116562 Rat
- SwissProt: P17108 Rat
- Unigene: 9871 Rat
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100769 は 1 報の論文で使用されています。
- Kadasa NM et al. Hepatoprotective effects of curcumin against diethyl nitrosamine induced hepatotoxicity in albino rats. Asian Pac J Cancer Prev 16:103-8 (2015). PubMed: 25640336