Rat IL-2 ELISA Kit (Interleukin-2) (ab100769)


  • 製品名Rat IL-2 ELISA Kit (Interleukin-2)
    IL2 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.1 ng/ml
  • 検出範囲
    0.12 ng/ml - 30 ng/ml
  • 添加回収試験

    85 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 78.52 67% - 89%
    Serum 98.73 88% - 109%
    Plasma 80.35 70% - 91%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-2 (Interleukin-2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in serum, plasma and cell culture supernatants. (Rat IL-2 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 関連疾患Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 配列類似性Belongs to the IL-2 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-2 ELISA Kit (Interleukin-2) 画像

  • Representative standard curve using ab100769

  • Representative standard curve using ab100769


Rat IL-2 ELISA Kit (Interleukin-2) (ab100769) 使用論文

ab100769 has not yet been referenced specifically in any publications.

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While ab100769was neither designed nor validated with any lysate samples, the kit is very accommodating to many different samples types s...

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