Rat IL-2 ELISA Kit (Interleukin-2) (ab100769)

製品の概要

  • 製品名
    Rat IL-2 ELISA Kit (Interleukin-2)
    IL2 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.1 ng/ml
  • 検出範囲
    0.12 ng/ml - 30 ng/ml
  • 添加回収試験

    85 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 78.52 67% - 89%
    Plasma 80.35 70% - 91%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-2 (Interleukin-2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in plasma and cell culture supernatants. (Rat IL-2 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat IL-2 in normal serum with ab100769, only in serum spiked with Rat IL-2.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100769

  • Representative standard curve using ab100769

プロトコール

参考文献

ab100769 has not yet been referenced specifically in any publications.

レビューと Q&A

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While ab100769was neither designed nor validated with any lysate samples, the kit is very accommodating to many different samples types s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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