Rat IL-13 ELISA Kit (Interleukin-13) (ab100766)

製品の概要

  • 製品名Rat IL-13 ELISA Kit (Interleukin-13)
    IL13 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    27.43 pg/ml - 20000 pg/ml
  • 添加回収試験

    114 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 120.4 80% - 139%
    Tissue Extracts 107.8 81% - 135%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.

    This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 関連疾患Allergic rhinitis
  • 配列類似性Belongs to the IL-4/IL-13 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-13 ELISA Kit (Interleukin-13) 画像

  • Representative standard curve using ab100766

プロトコール

Rat IL-13 ELISA Kit (Interleukin-13) (ab100766) 使用論文

ab100766 has not yet been referenced specifically in any publications.

Product Wall

ab100766 tested now on rat serum

Good Good 4/5 (Ease of Use)
Abreviews
the product worked perfectly on rat serum with the same protocol suggested in the kit for cell culture exctracts, tissue exctracts.
Username

Vasiliki Koumaki

Verified customer

投稿 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"