Rat IL-13 ELISA Kit (ab100766)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-13 ELISA Kit
IL-13 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
27.43 pg/ml - 20000 pg/ml -
添加回収試験
114 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture extracts 120.4 80% - 139% Tissue Extracts 107.8 81% - 135% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.
This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-rat IL-13 2 vials IL-13 Microplate (12 x 8 wells) 1 unit Recombinant Rat IL-13 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
関連疾患
Allergic rhinitis -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Allergic rhinitis
- ALRH
- BHR 1
see all -
参照データベース
- Entrez Gene: 116553 Rat
- SwissProt: P42203 Rat
- Unigene: 9921 Rat
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab100766 は 5 報の論文で使用されています。
- Jing L et al. Srolo Bzhtang, a traditional Tibetan medicine formula, inhibits cigarette smoke induced airway inflammation and muc5ac hypersecretion via suppressing IL-13/STAT6 signaling pathway in rats. J Ethnopharmacol 235:424-434 (2019). PubMed: 30731182
- Yan M et al. The Mechanism of Electroacupuncture at Zusanli Promotes Macrophage Polarization during the Fibrotic Process in Contused Skeletal Muscle. Eur Surg Res 60:196-207 (2019). PubMed: 31694021
- Iturra PA et al. Progression of Type 2 Helper T Cell-Type Inflammation and Airway Remodeling in a Rodent Model of Naturally Acquired Subclinical Primary Pneumocystis Infection. Am J Pathol 188:417-431 (2018). PubMed: 29169991
- Seiffert J et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . PubMed: 25747867
- Qin WY et al. Electroacupuncture Could Regulate the NF-?B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats. Evid Based Complement Alternat Med 2013:924541 (2013). PubMed: 23970940