製品の概要

  • 製品名
    Rat IL-13 ELISA Kit (Interleukin-13)
    IL13 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    27.43 pg/ml - 20000 pg/ml
  • 添加回収試験

    114 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 120.4 80% - 139%
    Tissue Extracts 107.8 81% - 135%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.

    This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100766

プロトコール

参考文献

This product has been referenced in:
  • Seiffert J  et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . Read more (PubMed: 25747867) »

See 1 Publication for this product

レビューと Q&A

ab100766 tested now on rat serum

Good Good 4/5 (Ease of Use)
Abreviews
the product worked perfectly on rat serum with the same protocol suggested in the kit for cell culture exctracts, tissue exctracts.
Username

Vasiliki Koumaki

Verified customer

投稿 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録