製品の概要

  • 製品名Rat IL-10 ELISA Kit (Interleukin-10)
    IL10 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 60 pg/ml
  • 検出範囲
    0.063 ng/ml - 4 ng/ml
  • 全工程の試験時間
    4h 30m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-10 (Interleukin-10) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in cell culture supernatants.

    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性Belongs to the IL-10 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-10 ELISA Kit (Interleukin-10) 画像

  • Representative Standard Curve Using ab108872.

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Rat IL-10 ELISA Kit (Interleukin-10) (ab108872) 使用論文

ab108872 has not yet been referenced specifically in any publications.

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