製品の概要

  • 製品名Rat IL-1 alpha ELISA Kit (Interleukin-1 alpha)
    IL1 alpha キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    11 pg/ml - 8000 pg/ml
  • 添加回収試験

    81 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 87.48 76% - 95%
    Serum 79.95 69% - 88%
    Plasma 75.73 68% - 84%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s IL-1 alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 alpha in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性IL1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • 細胞内局在Secreted
  • 別名
    • Hematopoietin 1
    • IL 1 alpha
    • Il 1a
    • IL1
    • IL1 ALPHA
    • IL1A
    • IL1F1
    • Ilia
    • Interleukin 1 alpha
    • Interleukin 1 alpha precursor
    • Interleukin1 alpha
    • OTTHUMP00000203692
    • OTTMUSP00000016430
    • Preinterleukin 1
    • Pro interleukin 1
    • Pro interleukin 1 alpha
    • Prointerleukin 1
    • RP23-160G19.8
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-1 alpha ELISA Kit (Interleukin-1 alpha) 画像

  • Representative standard curve using ab113350

プロトコール

Rat IL-1 alpha ELISA Kit (Interleukin-1 alpha) (ab113350) 使用論文

This product has been referenced in:
  • Zhang X  et al. Risk of radiation-induced pneumonitis after helical and static-port tomotherapy in lung cancer patients and experimental rats. Radiat Oncol 10:195 (2015). Read more (PubMed: 26382926) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"