Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

製品の概要

  • 製品名Rat IgG1, kappa monoclonal [RTK2071] - Isotype control
    Rat アイソタイプ・コントロール 製品一覧
  • アプリケーション適用あり: IHC-P, IHC-Fr, Flow Cyt, WB, IPmore details
  • 免疫原

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • 特記事項


    The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • アイソタイプ・コントロール 備考The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • ポリ/モノモノクローナル
  • クローン名RTK2071
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野
  • 別名
    • Rat Isotype Control

アプリケーション

Our Abpromise guarantee covers the use of ab18412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 2µg for 106 cells. ab18412 is a negative isotype control and can be used for surface or intracellular staining
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control 画像

  • Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412) 使用論文

ab18412 has not yet been referenced specifically in any publications.

Product Wall


Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
ab18412 (http://www.abcam.com/Rat-IgG1-kappa-mon...

Read More

Please find attached to this email the MSDS you requested for the following antibodies:
ab3104
ab18412
ab45139
ab68672

A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few...

Read More

Thank you for contacting us.

I would like to inform you that we are currently dealing with your request.

I will contact you again as soon as the requested documents are available.

Thank you for your patience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"