製品の概要

  • 製品名Rat Fas Ligand ELISA Kit (CD95L)
    Fas Ligand キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 90 pg/ml
  • 検出範囲
    82.3 pg/ml - 60000 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 92.64 81% - 98%
    Serum 123.4 118% - 135%
    Plasma 118.9 109% - 125%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s Fas Ligand (CD95L) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fas Ligand (CD95L) in serum, plasma, and cell culture supernatants. (Rat Fas Ligand concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Rat Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • 細胞内局在Cell Membrane (single-pass type II membrane protein) and Secreted
  • 別名
    • Apoptosis (APO 1) antigen ligand 1
    • Apoptosis antigen ligand
    • APT1LG1
    • APTL
    • CD178
    • CD95 L
    • CD95 ligand
    • CD95L
    • CD95L protein
    • Fas antigen ligand
    • FASL
    • FASLG
    • TNF superfamily member 6
    • TNFSF6
    • Tumor necrosis factor (ligand) superfamily member 6
    • Tumor necrosis factor ligand superfamily member 6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーショ�� Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat Fas Ligand ELISA Kit (CD95L) 画像

  • Representative Standard Curve using ab100759.

  • Representative Standard Curve using ab100759.

プロトコール

Rat Fas Ligand ELISA Kit (CD95L) (ab100759) 使用論文

ab100759 has not yet been referenced specifically in any publications.

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