Rat Ceruloplasmin ELISA Kit (CERP) (ab108819)

製品の概要

  • 製品名Rat Ceruloplasmin ELISA Kit (CERP)
    Ceruloplasmin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Urine
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.3 ng/ml
  • 検出範囲
    0.31 ng/ml - 20 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s Ceruloplasmin (CERP) rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 配列類似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Rat Ceruloplasmin ELISA Kit (CERP) 画像

  • Representative Standard Curve using ab108819.

プロトコール

Rat Ceruloplasmin ELISA Kit (CERP) (ab108819) 使用論文

ab108819 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and interest in our products.

Your customer can use the kit (ab108819) to determine cell lysate ceruloplasmin level, though it is advised to perform a serial dilution of the sample to determine what is the most opti...

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Thank you for your enquiry.

This kit has been tested on cell culture supernatant and urine as samples - but not on serum.

I would advise you to take a look at the datasheet and the Protocol Booklet for further information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"