Rat Ceruloplasmin ELISA Kit (CERP) (ab108819)

製品の概要

  • 製品名
    Rat Ceruloplasmin ELISA Kit (CERP)
    Ceruloplasmin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.4%
  • サンプルの種類
    Cell culture supernatant, Urine
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.3 ng/ml
  • 検出範囲
    0.31 ng/ml - 20 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s Ceruloplasmin (CERP) rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • Representative Standard Curve using ab108819.

プロトコール

参考文献

ab108819 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry and interest in our products.

Your customer can use the kit (ab108819) to determine cell lysate ceruloplasmin level, though it is advised to perform a serial dilution of the sample to determine what is the most opti...

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Thank you for your enquiry.

This kit has been tested on cell culture supernatant and urine as samples - but not on serum.

I would advise you to take a look at the datasheet and the Protocol Booklet for further information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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