Rat Ceruloplasmin ELISA Kit (CERP) (ab108819)
Key features and details
- Sensitivity: 74 ng/ml
- Range: 1 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat Ceruloplasmin ELISA Kit (CERP)
Ceruloplasmin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 8.3% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 74 ng/ml -
検出範囲
1 ng/ml - 10 ng/ml -
添加回収試験
95 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s Ceruloplasmin (CERP) rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.
A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat Ceruloplasmin Antibody 1 x 120µl Ceruloplasmin Microplate (12 x 8 well strips) 1 unit Ceruloplasmin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. -
配列類似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 6 plastocyanin-like domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CERU_HUMAN
- Ceruloplasmin
- CP
see all -
参照データベース
- Entrez Gene: 24268 Rat
- SwissProt: P13635 Rat
- Unigene: 32777 Rat
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108819 は論文での使用が確認できていません。