製品の概要

  • 製品名
    Rat C Reactive Protein ELISA Kit (PTX1)
    C Reactive Protein キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.6%
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.7 ng/ml
  • 検出範囲
    0.78 ng/ml - 50 ng/ml
  • 添加回収試験

    95 %

  • 全工程の試験時間
    3h 30m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma, serum, and cell culture supernatants.

    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab108827.

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参考文献

This product has been referenced in:
  • Xu J  et al. Optimized rapeseed oils rich in endogenous micronutrients ameliorate risk factors of atherosclerosis in high fat diet fed rats. Lipids Health Dis 13:166 (2014). Rat . Read more (PubMed: 25358951) »

See 1 Publication for this product

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