製品の概要

  • 製品名Rat BNP 45 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.03 ng/ml
  • 検出範囲
    0.031 ng/ml - 1 ng/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.

    A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.

  • 特記事項

    There is significant cross-reactivity between BNP32 and BNP45.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 組織特異性Brain and also in atria, but at much lower levels than ANP.
  • 配列類似性Belongs to the natriuretic peptide family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • BNP-45
    • Gamma-brain natriuretic peptide
    • nppb
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Rat BNP 45 ELISA Kit 画像

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted

  • Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample

プロトコール

Rat BNP 45 ELISA Kit (ab108816) 使用論文

This product has been referenced in:
  • Youcef G  et al. Simultaneous characterization of metabolic, cardiac, vascular and renal phenotypes of lean and obese SHHF rats. PLoS One 9:e96452 (2014). Read more (PubMed: 24831821) »
  • Wang YB  et al. Rehmannia glutinosa extract activates endothelial progenitor cells in a rat model of myocardial infarction through a SDF-1 a/CXCR4 cascade. PLoS One 8:e54303 (2013). ELISA ; Rat . Read more (PubMed: 23349848) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

Currently, this is the only BNP 45 ELISA kit (ab108816) which recognizes rat BNP 45. We sell primary antibodies which detect human BNP:

ab20984,

ab19645,

ab19646,

ab47699, Read More

Our samples showed around 40% cross-reactivity for rabbit protein.

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet êtr...

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Vielen Dank für Ihren Anruf.

Wenn das Futter keinen Einfluss auf die Produktion dieser Peptide hat (wie durch zB salzreiche Nahrung), sehe ich keinen Grund, warum die Ratten fasten sollten.

Ich hoffe, dies hilft Ihnen weiter....

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Thank you for contacting us.

The best way to test the viability is using the kit. The kits should be fine at -20C so please use the kit without any hesitation.

I hope this information is helpful to you. Please do not hesitate to conta...

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Thank you for your reply and the additional information.

We have the following PO on file: xxx.The order was from xxx and the lot number matches what you had sent, thus I think this is the correct order.


I have now arranged t...

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Thank you for contacting us.

I am very sorry to hear about the problems your customer had to face because of the second kit. I sincerely apologize about that! Yes, I'd be happy to arrange for a free of charge replacement.


Cou...

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Gracias por contactarnos y alertarnos de este fallo.



Lamento mucho que el vial de estándar del nuevo lote enviado no indicara ningún dato acerca de la cantidad contenida. Sin duda se trata de un error que estamos trata...

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Thank you for your enquiry and your interest.

As the Protocol booklet indicates we would suggest adding 50 μl of Chromogen Substrate per well and incubate for about 15 minutes or till the optimal blue colour density develops. The optimal t...

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Thank you for your enquiry.

I can confirm that the first difference between these kits is that ab108815 detects the 32 amino acid form of BNP, andab108816 detects the larger 45 amino acid form of the protein.

According to a litera...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"