製品の概要

  • 製品名Rat BNP 32 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Tissue
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.06 ng/ml
  • 検出範囲
    0.031 ng/ml - 2 ng/ml
  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s BNP 32 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 32 in plasma, serum, tissue extract, and cell culture supernatants.

    A BNP 32 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 32 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 32 captured in plate.

  • 特記事項

    There is significant cross-reactivity between BNP32 and BNP45.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 組織特異性Brain and also in atria, but at much lower levels than ANP.
  • 配列類似性Belongs to the natriuretic peptide family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Gamma-brain natriuretic peptide
    • nppb
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab108815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Rat BNP 32 ELISA Kit 画像

  • Representative Standard Curve using ab108815.

プロトコール

Rat BNP 32 ELISA Kit (ab108815) 使用論文

This product has been referenced in:
  • Hajje G  et al. Hypothyroidism and its rapid correction alter cardiac remodeling. PLoS One 9:e109753 (2014). Sandwich ELISA ; Rat . Read more (PubMed: 25333636) »

See 1 Publication for this product

Product Wall



Le laboratoire m’a confirmé que vous pouvez utiliser un tampon EDTA plutôt qu’un tampon de citrate pour vos échantillons de plasma.

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet êtr...

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Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Cou...

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Thank you for your enquiry.

I can confirm that the first difference between these kits is that ab108815 detects the 32 amino acid form of BNP, andab108816 detects the larger 45 amino acid form of the protein.

According to a litera...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"