• 製品名
    Rat ANP ELISA Kit (NPPA)
  • 検出方法
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 4.9%
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.2 ng/ml
  • 検出範囲
    0.25 ng/ml - 4 ng/ml
  • 全工程の試験時間
    5h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Pig
  • 製品の概要

    Abcam’s ANP (NPPA) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ANP concentrations in cell culture supernatants, tissue-extract, serum and plasma.

    An ANP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an ANP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of ANP captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Rat ANP Antibody 1 x 90µl
    ANP Microplate (12 x 8 well strips) 1 unit
    ANP Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野
  • 機能
    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 関連疾患
    Familial atrial fibrillation 6
  • 配列類似性
    Belongs to the natriuretic peptide family.
  • 翻訳後修飾
    Cleaved by CORIN upon secretion to produce the functional hormone.
  • 細胞内局在
  • Information by UniProt
  • 別名
    • ANF
    • ANP
    • ATFB6
    • Atrial natriuretic factor
    • Atrial natriuretic peptide
    • Atriopeptin
    • Cardionatrin
    • CDD ANF
    • CDD-ANF
    • CDP
    • Natriuretic peptide A
    • NPPA
    • PND
    • Prepronatriodilatin
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab108797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Rat ANP measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108797.



This product has been referenced in:
  • Powell M  et al. Assessment of endothelial glycocalyx disruption in term parturients receiving a fluid bolus before spinal anesthesia: a prospective observational study. Int J Obstet Anesth 23:330-4 (2014). Read more (PubMed: 25201316) »
  • Rössger K  et al. Reward-based hypertension control by a synthetic brain-dopamine interface. Proc Natl Acad Sci U S A 110:18150-5 (2013). ELISA ; Human . Read more (PubMed: 24127594) »

See all 2 Publications for this product

レビューと Q&A

Good product!

Excellent Excellent 5/5 (Ease of Use)
The ELISA Kit is easy to use and work well.
Rat plasma was used with five times dilution.(For reference)

Miss. Rui Ma

Verified customer

投稿 Jun 20 2017

We tested mouse samples only at two different concentrations, which is not ideal to verify linearity. We did get a significant signal, but there is not a satisfying reduction in signal at dilution 1/10 vs the neat sample. The rat values titrate out bet...

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The lab has testd several plasma and serum samples, all at no dilution (1x). The value may be 0.3 ng/ml for human plasma/serum, but it may be higher or lower depending on the sample. Each sample is different and some optimization may be requi...

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Human plasma and serum has been tested with this kit. Rat EDTA plasma has also been tested, so human EDTA plasma should work with the kit as well.

Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Cou...

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Thank you for your patience with this reply.
We would recommend doing a serial dilution to see which protein concentration will fall within the standard curve. Take a look at our sample preparation guide for additional information. http://www.abca...

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The amount of ANP in the vial of standard for the lot you have is 20 nanograms. Please contact us if you have other questions about the assay.

Thank you very much for your inquiry. I am very sorry for the delay.

We tried to find a concrete value to propose for the extraction kit, however this is not possible.We recommend that each laboratory does optimize the conditions, dilutions f...

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Thank you for your enquiry. I am sorry it has taken some time to confirm this information for you.

As discussed on the telephone, I am sorry that asfar as we are aware, ab108797ANP Rat ELISA kithas never been tested with samples from horse. ...

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Thank you for contacting Abcam regarding this issue. It is okay to use heparin coated tubes and avoid having to use the 0.1M sodium citrate. I hope this helps. If there is anything else that I can help you with please let me know.

1-10 of 11 Abreviews or Q&A