製品の概要

  • 製品名
    Rat alpha 2 Macroglobulin ELISA Kit
    alpha 2 Macroglobulin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
  • サンプルの種類
    Serum, Plasma, Other biological fluids
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    2.23 ng/ml
  • 検出範囲
    6.25 ng/ml - 400 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum > 85 % - %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat
  • 製品の概要

    Abcam’s alpha 2 Macroglobulin Rat ELISA Kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of alpha 2 Macroglobulin in rat biological samples. This kit is specific to the alpha 2 Macroglobulin and will not cross react with the closely related alpha 1 Macroglobulin. 

    In this assay the alpha 2 Macroglobulin present in samples reacts with the anti-alpha 2 Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-alpha 2 Macroglobulin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound alpha 2 Macroglobulin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of alpha 2 Macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 2 Macroglobulin in the test sample. The quantity of alpha 2 Macroglobulin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab157730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • alpha-2-macroglobulin measured in various samples showing quantitie (ng) per mL of tested sample

  • Representative standard curve using ab157730 alpha 2 Macroglobulin Rat ELISA Kit.

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参考文献

ab157730 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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