製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RASSF1
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide, conjugated to KLH, corresponding to a region within the internal sequence of Human RASSF1.

  • ポジティブ・コントロール
    • Human brain cortex and liver tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • 組織特異性
    Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • 配列類似性
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • 細胞内局在
    Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 123F2 antibody
    • Cardiac specific ras association domain family 1 protein antibody
    • cardiac-specific ras association domain family 1 protein, 123F2 antibody
    • NORE2A antibody
    • OTTHUMP00000195503 antibody
    • OTTHUMP00000195504 antibody
    • OTTHUMP00000195505 antibody
    • OTTHUMP00000195507 antibody
    • Pancreas specific ras association domain family 1 protein antibody
    • Ras association (RalGDS/AF 6) domain family member 1 antibody
    • Ras association domain-containing protein 1 antibody
    • RASF1_HUMAN antibody
    • Rassf1 antibody
    • RASSF1A antibody
    • RDA32 antibody
    • REH3P21 antibody
    • Tumor suppressor protein RDA32 antibody
    • WUGSC:H_LUCA12.5 antibody
    see all

Anti-RASSF1 antibody 画像

  • ab110900, at 3.75 µg/ml, staining RASSF1 in formalin fixed, paraffin embedded Human brain cortex tissue by Immunohistochemistry. A biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen was used to develop staining.
  • ab110900, at 3.75 µg/ml, staining RASSF1 in formalin fixed, paraffin embedded Human liver tissue by Immunohistochemistry. A biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen was used to develop staining.

プロトコール

Anti-RASSF1 antibody (ab110900) 使用論文

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