製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RASGRP2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 35-83 (VRMFLMMHPW YIPSSQLAAK LLHIYQQSRK DNSNSLQVKT CHLVRYWISA) of Human RASGRP2 (NP_722541)

  • ポジティブ・コントロール
    • Human fetal brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
  • 組織特異性
    Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
  • 配列類似性
    Belongs to the RASGRP family.
    Contains 2 EF-hand domains.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • 発生段階
    Expressed in fetal brain, lung, liver and kidney.
  • ドメイン
    The N-terminal Ras-GEF domain mediates association with F-actin.
  • 翻訳後修飾
    Isoform 2 is palmitoylated and myristoylated while isoform 1.
  • 細胞内局在
    Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium and DAG-regulated antibody
    • Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
    • Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
    • CalDAG-GEFI antibody
    • Cdc25 like protein antibody
    • Cdc25-like protein antibody
    • F25B3.3 kinase like protein antibody
    • F25B3.3 kinase-like protein antibody
    • GRP2_HUMAN antibody
    • HCDC25L antibody
    • RAS guanyl releasing protein 2 antibody
    • RAS guanyl-releasing protein 2 antibody
    • Rasgrp2 antibody
    see all

画像

  • Anti-RASGRP2 antibody (ab94865) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa

プロトコール

参考文献

ab94865 has not yet been referenced specifically in any publications.

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