製品の概要

  • 製品名Anti-RASGRP2 antibody
    RASGRP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RASGRP2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: GTLDLDKGCT VEELLRGCIE AFDDSGKVRD PQLVRMFLMM HPWYIPSSQL AAKLLHIYQQ SRKDNSNSLQ VKTCHLVRYW ISAFPAEFDL NPELAEQIKE , corresponding to amino acids 65-165 of Human RASGRP2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
    • 組織特異性Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
    • 配列類似性Belongs to the RASGRP family.
      Contains 2 EF-hand domains.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • 発生段階Expressed in fetal brain, lung, liver and kidney.
    • ドメインThe N-terminal Ras-GEF domain mediates association with F-actin.
    • 翻訳後修飾Isoform 2 is palmitoylated and myristoylated while isoform 1.
    • 細胞内局在Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Calcium and DAG-regulated antibody
      • Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
      • Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
      • CalDAG-GEFI antibody
      • Cdc25 like protein antibody
      • Cdc25-like protein antibody
      • F25B3.3 kinase like protein antibody
      • F25B3.3 kinase-like protein antibody
      • GRP2_HUMAN antibody
      • HCDC25L antibody
      • RAS guanyl releasing protein 2 antibody
      • RAS guanyl-releasing protein 2 antibody
      • Rasgrp2 antibody
      see all

    Anti-RASGRP2 antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab56658 RASGRP2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-RASGRP2 antibody (ab56658) 使用論文

    This product has been referenced in:
    • Robert P  et al. A novel leukocyte adhesion deficiency III variant: kindlin-3 deficiency results in integrin- and nonintegrin-related defects in different steps of leukocyte adhesion. J Immunol 186:5273-83 (2011). WB ; Human . Read more (PubMed: 21441448) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab56658.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"