製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RASGRP 4
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 108 - 170 (KATGDTQELR RLQICHLVRY WLMRHPEVMH QDPQLEEVIG RFWATVAREG NSAQRRLGDS SDL) of Human RASGRP 4 (NP_733749).

  • ポジティブ・コントロール
    • WB: A431, H1299, HeLa and Raji cell lysates IHC-P: SW480 (Human colon adenocarcinoma) tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.

ターゲット情報

  • 機能
    Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
  • 組織特異性
    Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
  • 配列類似性
    Belongs to the RASGRP family.
    Contains 1 EF-hand domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • 発生段階
    Expressed early during development. Detected in all fetal tissues tested.
  • ドメイン
    The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.
  • 細胞内局在
    Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GRP4_HUMAN antibody
    • Guanyl nucleotide releasing protein 4 antibody
    • RAS guanyl releasing protein 4 antibody
    • RAS guanyl-releasing protein 4 antibody
    • RASGRP4 antibody
    see all

画像

  • ICC/IF image of ab96293 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96293 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RASGRP 4 antibody (ab96293) at 1/1000 dilution + Raji whole cell lysates at 30 µg

    Predicted band size : 75 kDa
    7.5% SDS PAGE
  • ab96293, at a 1/100 dilution, staining RASGRP 4 in paraffin embedded SW480 (Human colon adenocarcinoma) tissue by Immunohistochemical analysis.

参考文献

ab96293 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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