Anti-RASGRP 4 抗体 (ab96293)
Key features and details
- Rabbit polyclonal to RASGRP 4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RASGRP 4 antibody -
製品の詳細
Rabbit polyclonal to RASGRP 4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 108 - 170 (KATGDTQELR RLQICHLVRY WLMRHPEVMH QDPQLEEVIG RFWATVAREG NSAQRRLGDS SDL) of Human RASGRP 4 (NP_733749).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96293の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
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WB |
1/500 - 1/3000. Predicted molecular weight: 75 kDa.
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特記事項 |
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IHC-P
1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 75 kDa. |
ターゲット情報
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機能
Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation. -
組織特異性
Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient. -
配列類似性
Belongs to the RASGRP family.
Contains 1 EF-hand domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain. -
発生段階
Expressed early during development. Detected in all fetal tissues tested. -
ドメイン
The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG. -
細胞内局在
Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG. - Information by UniProt
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参照データベース
- Entrez Gene: 115727 Human
- Omim: 607320 Human
- SwissProt: Q8TDF6 Human
- Unigene: 130434 Human
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別名
- GRP4_HUMAN antibody
- Guanyl nucleotide releasing protein 4 antibody
- RAS guanyl releasing protein 4 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab96293 は 3 報の論文で使用されています。
- Tang W et al. RasGRP Exacerbates Lipopolysaccharide-Induced Acute Kidney Injury Through Regulation of ERK Activation. Open Forum Infect Dis 9:ofac041 (2022). PubMed: 35198649
- Zhu L et al. The critical role of RasGRP4 in the growth of diffuse large B cell lymphoma. Cell Commun Signal 17:92 (2019). PubMed: 31409422
- Chen X et al. RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma. Cancer Cell 31:685-696.e6 (2017). PubMed: 28486107